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Catalog# Product Description |
ICP9950 Ractopamine is an adrenergic beta-agonist which is banned from use as a feed additive. The anti-ractopamine antibody is produced in rabbit using ractopamine-KLH conjugates and affinity purified using ractopamine-agarose. The antibody was proven to be useful for detection and quantization of ractopamine in ELISAs and gold colloidal strip systems. |
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Indirect competitive ELISA for ractopamine: 1) Immobilized antigen ractopamine-BSA (EDC coupling): 0.25 mg/mL 1st Ab: 0.01ng/mL, 2nd Ab: 0.25 µg/mL Sensitivity: IC50 = 1.5 ng/mL free ractopamine Linear range: 0.5-15 ng/mL
Note: This is not an optimized system. Customer has to optimize their own assay system.
Immunogen Structure and Linked Position
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Rabbit
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Formulation
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500 µg/mL in PBS with 0.01% NaN3
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Immunogen
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Ractopamine KLH conjugates
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Purification
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Affinity purified with ractopamine on agarose
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Specificity
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Reacts specifically with ractopamine.
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Applications
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ELISA; gold colloid strip
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.
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